P44 REDUCTION OF INGROWN HAIRS AND MODIFICATION OF BEARD HAIR MORPHOLOGY IN AFRICAN-AMERICAN MEN WITH PSEUDOFOLLICULITIS BARBAE (PFB) TREATED WITH TOPICAL APPLICATION OF 13.9% EFLORNITHINE HCL (VANIQATM)
Shander D, Ahluwalia G, Funkhouser M, Coble D
Department Dermatology Research, Gillette Co. Technical Center, Needham MA, USA

An open label study conducted in African -American men with PFB who were topically treated with VaniqaTM for 16 weeks demonstrated clinically significant improvements in PFB associated with changes in hair follicle structure. The purpose of the study was to extend the observations of the above study to include analysis of ingrown low lying hairs (IGLLHs) and histological changes in beard fibers. IGLLHs are embedded in the grooves and rugosities of the skin and are predisposed to enter the skin and provoke inflammatory lesions. Treatments for decreasing the population of IGLLHs of offer options to improve the PFB condition. Facial surface replicas of 48 hour beard growth were obtained at the beginning and end of the 16 week treatment period with VaniqaTM and processed to characterize changes in the number and percentage of IGLLHs. The results demonstrated a significant (44%) reduction of IGLLHs at the end of treatment as well as an increase in the average angle of emergence of beard hair measured by image analysis. Beard hair samples were also collected at the beginning and end of treatment and cross sections were prepared at a thickness of two microns. Before treatment the beard hairs had a triangular shape and at post treatment beard fibers assumed a multi-lobular shape and a significant increase in total fiber cross sectional area and cuticle thickness. There was a decrease in the percentage of methylene blue stained paracortical cells from 90 % to 76% (p-value<0.001). The percentage of eosin stained orthocortical cells concurrently increased resulting in a striking visual change in cortical staining pattern at the end of treatment. Changes in the ratio of orthocortex to paracortex by VaniqaTM may modify the biophysical properties of beard hairs to impact their shape and/or angle of emergence after exiting the follicle orifice thereby reducing IGLLHs.